arXiv:2605.09994v2 Announce Type: replace
Abstract: Modern Large Foundation Model (LFM) training has transformed the data pipeline from a static ingestion layer into a dynamic component that must co-evolve with the training process. Existing systems are ill-equipped: colocated dataloaders offer no failure isolation, while message queue-based disaggregated dataloaders operate on a record/offset abstraction that cannot express the batch-level semantics required by distributed training. We present BatchWeave, an object-store-native training data plane for distributed LFM training. BatchWeave uses versioned manifests and conditional object writes to coordinate batch publication, recovery, and lifecycle management. First, it introduces the Transactional Global Batch (TGB), which builds on versioned-manifest ACID storage semantics and extends them with training-specific consistency, including atomic all-rank batch visibility, a globally ordered step sequence, checkpoint-aligned lifecycle management, and end-to-end exactly-once recovery. Second, it realizes recovery and retention directly in the storage layer, by durably persisting producer state through the commit protocol and tying reclamation to distributed checkpoint state. Third, its Decentralized Adaptive Commit (DAC) algorithm sustains stable ingestion throughput as the manifest grows, without any inter-producer communication. Evaluations on large-scale multimodal pre-training and SFT workloads using 64 GPUs show that BatchWeave outperforms colocated dataloader throughput while providing full failure isolation, outperforms Apache Kafka in ingestion throughput, and achieves lower consumer read latency than Kafka.
Science Journals
arXiv:2605.10052v2 Announce Type: replace
Abstract: As artificial intelligence engineering paradigms shift from single-agent Prompt and Context Engineering toward multi-agent \textbf{Coordination Engineering}, the ability to codify and systematically improve how multiple agents collaborate has emerged as a critical bottleneck. While single-agent skills can now be distributed as portable assets, multi-agent coordination protocols remain locked within framework-internal code or static configurations, preventing them from being shared across systems or autonomously improved over time. We propose \textbf{Swarm Skills}, a portable specification that extends the Anthropic Skills standard with multi-agent semantics. Swarm Skills turns multi-agent workflows into first-class, distributable assets that consist of roles, workflows, execution bounds, and a built-in semantic structure for self-evolution. To operationalize the specification's evolving nature, we present a companion self-evolution algorithm that automatically distills successful execution trajectories into new Swarm Skills and continuously patches existing ones based on multi-dimensional scoring (Effectiveness, Utilization, and Freshness), eliminating the need for human-in-the-loop oversight during the refinement process. Through an architectural compatibility analysis and a comprehensive qualitative case study using the open-source JiuwenSwarm reference implementation, we demonstrate how Swarm Skills achieves zero-adapter cross-agent portability via progressive disclosure, enabling agent teams to self-evolve their coordination strategies without framework lock-in.
arXiv:2605.15786v1 Announce Type: new
Abstract: We present a new strategic voting model where we use uncertainty representation to model preferences. Specifically, we use probability sets as uncertainty representations, together with lower and upper expected utility gains to take strategic decisions. Focusing on belief functions in particular, we demonstrate that this very expressive model includes in one sweep many existing models based on probabilities, sets or incomplete preferences. Additionally, we generalize several well-known convergence results from the literature to this broader representational setting. Furthermore, we illustrate how this model can capture more realistic scenarios for practical applications but also raises theoretical challenges.
arXiv:2605.16090v1 Announce Type: new
Abstract: Large vision-language models (LVLMs) have emerged as a powerful paradigm for multimodal intelligence, but their growing deployment also expands the attack surface of prompt injection. Despite this growing concern, existing attacks still suffer from a critical limitation: the injected prompt for one modality only steers the model's interpretation of that singular input. Alternatively, these attacks remain multimodal but fail to achieve cross-modal prompt perturbation. To bridge this gap, we introduce a novel cross-modal prompt injection attack CrossMPI, which can steer the model's interpretation of both textual and visual inputs via image-only prompt injection. Our design is underpinned by the following key breakthroughs. First, we turn the focus of the injected prompt perturbation optimization from the visual embedding space (typically with only $10^5$ parameters) to the model hidden state space (for multimodal information integration and with $10^7$ parameters). Then, two strategies are adopted to mitigate the optimization challenges posed by the larger parameter space. To constrain the optimized model parameter space, we introduce a layer selection strategy that identifies the layers most critical to multimodal integration. Interestingly, deviating from the past experience, our analysis reveals that the optimal layers for LVLM prompt perturbation reside in the middle of the model rather than the last. To constrain the image perturbation space, we propose a new distance-decremental perturbation budget assignment strategy that allocates budgets decrementally as the pixel distance to semantic-critical regions increases. Extensive experiments across multiple LVLMs and datasets show that our method significantly outperforms baseline approaches.
arXiv:2605.15787v1 Announce Type: new
Abstract: Why does a Transformer that has memorized its training set wait thousands of steps before it generalizes? Existing accounts locate this delay in norm minimization, feature emergence, or the late discovery of sparse subnetworks. These explanations capture important parts of the transition, but ignore a constraint unique to attention-based models: if attention discards an informative token, no bounded downstream computation can recover it. We formalize attention as an implicit Bayesian posterior over the task dependency graph and prove that generalization requires two separable conditions: a familiar Goldilocks bound on MLP capacity, coinciding with norm-based theories of grokking, and a novel Bayesian structural condition requiring attention to place sufficient mass on every informative token. This decoupling explains delayed generalization as delayed structural inference. Early in training, the MLP memorizes through unaligned features, drives the cross-entropy loss near zero, and thereby starves attention of structural gradient. Weight decay must then erode memorization before the missing graph becomes learnable, yielding the known inverse-weight-decay delay, which we derive as a structural waiting time. We then prove that this explaining-away delay can be bypassed by a KL-based structural intervention, yielding an inverse-intervention-strength scaling law for the grokking time. Experiments on algorithmic sequence tasks isolate structure from capacity and show that this Bayesian ticket matches or outperforms lottery-ticket transfer.
arXiv:2605.15788v1 Announce Type: new
Abstract: Proactive autoscaling for containerized workloads depends on knowing the provisioning delay, i.e., the time between a scaling decision and the moment new capacity is ready to serve traffic. In practice, this cold-start duration can vary substantially across environments and even across consecutive scale-out events. We present ADAPT (Adaptive Duration Approximation for Predictive Timing), an online EWMA estimator that tracks coldstart duration at runtime. ADAPT feeds a dynamic planning horizon, FH-OPT, into a Model Predictive Controller (MPC) that optimizes replica counts over a rolling window. Together, these components form a closed-loop proactive autoscaling design that adapts its lookahead based on measured provisioning delay. Evaluated across three policies (MPC+LSTM, MPC+Prophet, HPA) and six workload archetypes with five random seeds, MPC+LSTM achieves below 5% SLA violation on all workloads, compared with 7-19% for reactive HPA and up to 28.7% for MPC+Prophet on bimodal traffic.
arXiv:2603.24338v2 Announce Type: replace-cross
Abstract: Interleaved ADCs are critical for applications requiring multi-gigasample per second (GS/s) rates, but their performance is often limited by offset, gain, and timing skew mismatches across the sub-ADCs. We propose exact but compact expressions that describe the impact of each of those non-idealities on the output spectrum. We derive the distribution of the power of the induced spurs and replicas, critical for yield-oriented derivation of sub-ADC specifications. Finally, we provide a practical example in which calibration step sizes are derived under the constraint of a target production yield.
arXiv:2605.15666v1 Announce Type: new
Abstract: Hyperspectral imaging (HSI) provides dense spectral information for the Earth's surface, enabling material-level understanding of land cover and ecosystem dynamics. Despite recent progress in hyperspectral self-supervised learning (SSL), existing datasets remain temporally shallow, limiting the development of long-horizon spatiotemporal modeling. To address this gap, we introduce ChronoEarth-492K, the first large-scale, temporally calibrated hyperspectral SSL dataset built upon NASA's EO-1 Hyperion mission, the world's longest continuous hyperspectral archive up to date (2001-2017). ChronoEarth-492K comprises 492,354 radiometrically harmonized patches across 185,398 global locations over 17 years, with 28,786 sites containing multi-temporal sequences ($\geq 3$ observations) that enable both short- and long-horizon temporal analysis. Building on this foundation, we establish the ChronoEarth-Benchmark, a unified evaluation suite spanning static, short-horizon, and long-horizon temporal tasks, constructed from six open-source geospatial products covering land cover, crop type, forest dynamics, and soil properties. We further introduce a standardized evaluation protocol and report extensive baseline results across state-of-the-art hyperspectral foundation models. Together, ChronoEarth and benchmark provide the first large-scale, temporally grounded platform for systematic spatiotemporal hyperspectral representation learning.
arXiv:2605.15935v1 Announce Type: new
Abstract: Plasma shape control in tokamaks requires a real-time controller that tracks dynamically changing shape targets while tolerating diagnostic failures. Classical approaches decompose the problem into equilibrium reconstruction followed by a linear controller, and assume a fixed, fully operational sensor set. We present a reinforcement learning agent that addresses both limitations simultaneously. The agent is trained in NSFsim, a high-fidelity tokamak simulator configured for DIII-D, on a curated dataset of 120 experimental plasma shapes. The shape targets are resampled as random step changes every 0.25 s, exposing the agent to diverse transitions across the full shape envelope. At test time the agent zero-shot tracks dynamic shape sequences; on a held-out static configuration in simulation it achieves a mean shape error of 2.01 cm, and dynamic trajectory following is demonstrated qualitatively in simulation and on the physical device. Diagnostic dropout randomly masks 30% of magnetic sensors per episode, yielding a single policy robust to arbitrary sensor subsets without backup controllers or mode-switching logic. An asymmetric actor-critic architecture with privileged equilibrium information improves value estimation under partial observability; an auxiliary shape reconstruction head on the actor enables end-to-end shape reconstruction from raw diagnostics and serves as an interpretability tool for policy analysis. The policy transfers to experimental DIII-D shots, where it directly commands the coil actuators on two dynamic shape maneuvers, and to the independent GSevolve simulator.
arXiv:2605.10057v3 Announce Type: replace
Abstract: Compositional spatiotemporal reasoning often requires a system to invoke multiple heterogeneous specialists, such as geometric, temporal, topological, and trajectory agents. A central question is how such a system should route among specialists when execution does not simply succeed or fail, but fails in qualitatively different ways. Existing tool-augmented and multi-agent LLM systems typically leave this routing decision implicit in language generation, making recovery ad hoc, difficult to interpret, and hard to optimize. This paper presents STAR (Spatio-Temporal Agent Router), a failure-aware routing framework that externalizes inter-agent control as a state-conditioned transition policy over the current agent, task type, and typed execution status. At the center of STARis an agent routing matrix that combines expert-specified nominal routes with recovery transitions learned from execution traces. Because the matrix conditions on distinct failure states, the router can respond differently to malformed outputs, missing dependencies, and tool--query mismatches, rather than collapsing them into a generic retry signal. Specialists execute through a tool-grounded extract--compute--deposit protocol and write intermediate results to a shared blackboard for downstream fusion. Results prove that retaining unsuccessful traces during training enlarges the support of the routing policy on error states, enabling recovery transitions that success-only training cannot represent. Across three spatiotemporal benchmarks and eight backbone LLMs, STAR improves over multiple baselines with the clearest gains on queries whose execution deviates from the nominal routing path. Router-specific ablations and recovery analyses further show that typed failure-aware routing, rather than specialist composition alone, is a key factor for these improvements.
arXiv:2605.15792v1 Announce Type: new
Abstract: The long-standing goal of multimodal AI is to build unified models in which visual understanding and visual generation mutually enhance one another. Despite recent works such as BAGEL, BLIP3o achieves remarkable progress; In practice, however, this unification remains one-directional: understanding routinely guides generation, yet how and why generation can support understanding is rarely investigated. We revisit this asymmetry and propose Generation-to-Understanding (G2U) synergy, where visual generation becomes an explicit intermediate reasoning step. Our framework enables a model to perform controlled generative acts, such as detail enhancement, context expansion or structural visualisation, to produce self-generated visual thoughts, which are then fed back into the model to refine perception without retraining or external tools. Through a comprehensive evaluation on twelve benchmarks, this reversed information flow consistently improves multimodal understanding. We show that generative fidelity bounds perceptual gain and that distinct families of edit prompts govern transfer efficiency. We further analyse whether models can decide what to imagine. While they can produce plausible edits, these self-generated visual thoughts lack stable task alignment, revealing that current large multimodal models fall short of true self-reflection. This work exposes a missing mechanism in unified cognition and suggests that imagination is not the end of understanding but its beginning.
arXiv:2605.15950v1 Announce Type: new
Abstract: This work extends the generalized nearest neighbor decoding (GNND), originally developed as a receiver architecture for memoryless channels, to a vectorized GNND (Vec-GNND) suitable for in-block memory (IBM) channels. Leveraging the generalized mutual information (GMI) as an operational lower bound on the mismatch capacity, an analytical characterization of the optimal Vec-GNND is obtained for general IBM channels with Gaussian codebooks. The formalism further provides closed-form optimality conditions and achievable GMIs for restricted variants of the receiver architecture. Furthermore, we formulate a GMI-based joint design viewpoint for Gaussian codebook covariance and decoding metrics. Since the metric optimization admits a closed-form solution for each fixed covariance, the joint design is reduced to an input-covariance optimization problem; for the diagonal covariance family, we derive first-order self-consistent optimality conditions. Numerical evaluations on block noncoherent additive white Gaussian noise channels and phase noise channels demonstrate consistent performance gains over conventional scaling-based baselines, highlighting the substantial advantages and potential relevance of the proposed Vec-GNND in realistic communication scenarios.
arXiv:2602.23892v2 Announce Type: replace-cross
Abstract: Fully Probabilistic design (FPD) is a powerful framework offering an elegant and unifying account of stochastic control, learning and decision-making. Here we introduce a generalized FPD framework, which we term as Tsallis FPD. Tsallis FPD uses Tsallis divergence in place of the Kullback-Leibler divergence that defines the standard FPD cost term. Tsallis divergence is a natural generalization of the KL divergence, rooted in non-extensive statistical mechanics and providing flexibility towards modeling stochastic processes with non-Gaussian tail behavior. After formulating Tsallis FPD, we develop a constructive proof of convergence by formulating a fixed point iteration. The construction takes the form of a double iteration scheme that performs a sequence of backwards inductions, rather than a single pass down the stages that constitutes the proven approach for classical FPD. We prove that this construction asymptotically converges to a fixed point and that this fixed point is an optimal solution to Tsallis FPD.
arXiv:2605.15793v1 Announce Type: new
Abstract: Pre-training neural operators on diverse partial differential equation (PDE) datasets has emerged as a promising direction for building general-purpose surrogate models in scientific machine learning. However, the inherent complexity and structural diversity of PDE solution operators make multi-PDE pre-training fundamentally challenging. Existing methods mainly address this by increasing model capacity, while leaving the target solution operators unchanged. Inspired by classical numerical analysis, we instead propose to transform complex and diverse solution operators into simpler, better-aligned forms that are easier to model jointly. Since the optimal transformation varies across PDE types, it must be adaptive and input-dependent, allowing a single neural operator to approximate an entire family of operators. We instantiate this idea as AOT-POT (adaptive operator-transformation for pre-training operator transformer), which expands hidden representations into multiple parallel streams, adaptively aggregates and redistributes them before and after each sub-layer, and mixes streams through Sinkhorn-projected doubly stochastic matrices for stable training. These mechanisms together reshape diverse solution operators into a unified form that can be effectively modeled by a single architecture. Empirically, AOT-POT achieves state-of-the-art performance on 12 PDE benchmarks with only 3\% additional parameters, reducing relative L2 error by up to 77.6\% (40.9\% on average). Fine-tuning AOT-POT further reduces L2 error by up to 92\% on in-domain PDEs and 89\% on out-of-domain PDEs (unseen types during pre-training), demonstrating that adaptive operator transformation is an effective and complementary direction for advancing PDE foundation models beyond simply scaling model capacity.
arXiv:2605.15951v1 Announce Type: new
Abstract: Finetuning Large Vision-Language Models with reinforcement learning has emerged as a promising approach to enhance their capability in object-level grounding. However, existing methods, mainly based on GRPO, assign rewards at the response level. Such sparse reward, often criterion-induced, leads to minimal learning signals when all candidate responses fail in challenging scenarios. In this work, we propose a group-revision optimisation paradigm that enhances learning on hard cases. It begins with a sampled initial response and generates a set of revised candidates to explore improved grounding outcomes. Inspired by reward shaping, we introduce a consolidation process that quantifies each candidate's improvement over the initial attempt and converts it into informative shaping signals. These signals are used to both refine the reward and modulate the advantage, amplifying the influence of high-quality revisions. Our method achieves consistent gains across referring and reasoning segmentation, REC, and counting benchmarks compared with prior GRPO-based models. Our code is available at https://github.com/yyliu01/GroupRevision.
arXiv:2605.16208v1 Announce Type: cross
Abstract: Flexible continuous-time survival modeling is critical for capturing complex time-varying hazard dynamics in high-dimensional data; however, training such models remains challenging due to the intractable integral required for likelihood estimation. We introduce QSurv, a scalable deep learning framework that enables nonparametric continuous-time modeling without relying on time discretization or restrictive distributional assumptions. We propose a training objective based on Gauss-Legendre numerical quadrature, which approximates the cumulative hazard with high-order accuracy while facilitating efficient end-to-end training via standard backpropagation. Furthermore, to effectively capture non-stationary hazard dynamics in complex architectures, we introduce time-conditioned low-rank adaptation, a mechanism that conditions general neural backbones on time by dynamically modulating weights via low-rank updates. We provide theoretical analysis establishing approximation error bounds for cumulative-hazard evaluation. Comprehensive experiments across synthetic benchmarks, large-scale real-world tabular datasets, and high-dimensional medical imaging tasks demonstrate that QSurv achieves competitive predictive performance with advantages in instantaneous hazard function estimation, enabling more interpretable characterization of time-varying risk patterns.
arXiv:2605.13143v2 Announce Type: replace
Abstract: Knowledge distillation is widely used to improve generalization in practice, yet its theoretical understanding remains elusive. In the standard distillation setting, a teacher model provides soft predictions to guide the training of a student model. We model teacher and student training as coupled stochastic processes and introduce a distillation divergence, defined as the Kullback-Leibler divergence between these two stochastic kernels. Within this framework, we derive two generalization bounds for the student model relative to the teacher's generalization gap: an upper bound under a sub-Gaussian assumption via algorithmic stability, and a lower bound under a central condition with sharper dependence on the distillation divergence. We further develop a loss-sharpness-aware bound with an explicit tightness regime, showing that the teacher's local flatness can strictly tighten the bound. Additionally, in a linear Gaussian case study, the distillation divergence admits an interpretable decomposition into bias, variance, and rank-bottleneck costs, yielding practical guidance for distillation design.
arXiv:2605.15754v1 Announce Type: new
Abstract: Driven by rapid advances in artificial intelligence and modern GPU computing capabilities, deep learning methods based on the optimization paradigm have provided new pathways to solve spatiotemporal physical problems, whose mathematical core lies in solving partial differential equations (PDEs). As an emerging class of function-space learning methods, neural operators (NOs) have exhibited great potential in efficient PDE solving. However, existing mainstream neural operator frameworks suffer from critical bottlenecks when modeling time-dependent PDEs over long time horizons, including accuracy degradation, insufficient stability, high training costs, and excessive memory consumption, which severely limit their practical deployment. To address these challenges in long-time prediction with neural operators, we propose a novel spatiotemporally decoupled physics-informed neural operator architecture, termed the physics-informed Stone-Weierstrass neural operator (PI-SWNO). The design is theoretically grounded in the decoupling paradigm combining time-invariant spatial basis functions with time-varying evolution coefficients, as well as the Stone-Weierstrass approximation theorem. By encoding spatial and temporal information via two separate subnetworks, the framework structurally mitigates the accumulation of errors over extended time intervals. Furthermore, we introduce a time-marching batch-wise sampling strategy to resolve the memory bottleneck of full-range modeling over extended time spans, ensuring continuity and convergence of full-time-domain solutions.
arXiv:2605.15996v1 Announce Type: cross
Abstract: We consider the problem of testing properties of graphs underlying high-dimensional graphical models. We adopt the model of covariance queries introduced by Lugosi, Truszkowski, Velona, and Zwiernik (2021). We study the case when the underlying graph is a tree. The main results of the paper show that, while reconstructing the entire tree may be costly, certain global structural properties can be tested efficiently. In particular, we design randomized tests for global structural properties that use a sub-quadratic number of queries. We develop testing procedures for several fundamental properties, including the number of leaves, the maximum degree, the typical distance, and the diameter of the tree. For each property, we obtain explicit query complexity bounds that depend on the target threshold and tolerance parameters.
arXiv:2605.15957v1 Announce Type: new
Abstract: Vector search (VS) is now available in most database engines. However, while vector search is a common feature in AI/ML/LLMs where the dominant computing platforms are GPUs, existing database engines operate on CPUs even when implementing vector search. This raises the question of whether integrating vector processing on GPUs as part of the engine would be a better design. In this paper, we explore this question in detail. First, we extend the TPC-H benchmark with vector data (from text and images) and propose a number of representative SQL+VS queries. Second, we develop a modular execution engine that can run SQL+VS queries across CPU and GPU. Third, we perform extensive experiments on a number of deployments: running the SQL+VS queries across CPU and/or GPU, with data residing in CPU or GPU memory, with existing indices and novel, optimized versions, as well as across different GPUs and interconnects (PCIe, NVLink). The results provide actionable and counter-intuitive insights on how to run such queries over CPUs and GPUs. For instance, the relational components benefit much more from running on the GPU than the vector search part. In addition, when the vector search involves moving data and indexes, using the GPU is not the best option, even with fast interconnects. Thus, we develop an alternative organization of vector index and embeddings that reduces the size of the index, making GPU-based vector search more competitive. With these improvements, the final result is that both the relational and vector search components are faster on the GPU, particularly on fast interconnects, in contrast with the architecture used in existing engines.
arXiv:2605.15794v1 Announce Type: new
Abstract: We present ForMaT (Format-Preserving Multilingual Translation), a parallel corpus of 3,956 PDFs across 15 language pairs that preserves original layout metadata proposed for multimodal machine translation. To ensure structural diversity in the dataset, we employ K-Medoids sampling over 45 geometric features, capturing complex elements like nested tables and formulas to focus only on visually diverse PDF documents. Our evaluation reveals that current MT systems struggle with spatial grounding and geometric synchronization, often losing the link between text and its visual context. ForMaT provides a benchmark for developing layout-aware translation models that integrate visual and textual context for high-fidelity document reconstruction.
arXiv:2605.15985v1 Announce Type: cross
Abstract: We introduce thermodynamic networks, a general framework for autonomous, physics-based computation using non-equilibrium steady states. These networks are modeled as a collection of finite-size reservoirs that exchange conserved quantities--such as electric charge or molecular number--while relaxing to a non-equilibrium steady state, which encodes the solution of a computational problem. We identify Negative Differential Conductance (NDC) as the critical physical property governing the computational expressivity of the thermodynamic network. While networks lacking NDC are restricted to computing monotonic functions, the presence of NDC enables universal function approximation. For the training of the network, we use protocols that take advantage of the natural tendency of the system to equilibrate. We illustrate the versatility of our approach via two different platforms: quantum dot networks and enzymatic reaction networks. Both systems can be engineered to have NDC, enabling high performance in standard benchmarks, including sine function approximation and MNIST digit classification. Overall, our work establishes a rigorous link between non-equilibrium steady states and computational expressivity.
arXiv:2605.15798v1 Announce Type: new
Abstract: Complex systems display emergent phenomena that vary significantly across spatial and temporal scales. These variations originate from fine-grained system processes, yet arriving at macroscopic dynamics from micro-level data -- particularly when large, high-resolution datasets are available -- remains a persistent challenge. Here we develop event-based spatiotemporal networks, a computational modelling framework that encodes system processes as discrete events anchored in space and time. Event-based spatiotemporal networks offer a unified, flexible and efficient approach to generate emergent behaviour in complex systems across space and time from these events. We demonstrate the effectiveness of event-based spatiotemporal networks through two illustrative real-world applications. First, following a local outbreak of a novel respiratory pathogen in the Netherlands, spatiotemporal networks enable fine-grained tracking of transmission routes and infection patterns through space and time. Second, we use spatiotemporal networks to model propagation of delays in a public transportation system (S-bahn) around Z\"urich, Switzerland. We also discuss broader uses of event-based spatiotemporal networks in fields like developmental biology and community ecology, where focusing on events rather than static system states can improve data analysis, simulation, and collection strategies.
arXiv:2605.15912v1 Announce Type: cross
Abstract: Subcellular protein complexes and organelles exhibit diverse dynamic behaviors that reflect the mechanical constraints and organization of the intracellular environment. Although some structures follow classical Brownian motion, many display anomalous dynamics. The transitions between these regimes are increasingly recognized as critical for subcellular organization, yet how they influence pattern formation remains unclear. Here, we investigate the spatial arrangement of cilia on the apical surface of multiciliated cells (MCCs) in developing Xenopus laevis embryos, where coordinated ciliary beating depends on the precise organization of hundreds of centriole-derived basal bodies (BBs). Using quantitative confocal, high-resolution and high-speed TIRF imaging together with theoretical modeling, we show that BB trajectories undergo time-resolved transitions between diffusive and anomalous motion, with distinct regimes that correlate with apical surface expansion. During the early stages, actin remodeling facilitates the dispersal of BBs by providing a permissive, low-confinement environment. As development progresses, the actin network becomes increasingly cross-linked that constrains BB movement and promotes uniform spacing across the apical domain. Disruption of $\alpha$-actinin-1, a major actin cross-linking protein, impairs the integrity of the apical actin meshwork, weakens BB confinement, and disrupts regular spatial patterning, ultimately compromising the arrangement of BBs required for proper cilia alignment. Together, we show that progressive apical actin cross-linking coordinates BB positioning and regulates their dynamic state, guiding the shift from diffusive to confined motion. This transition in dynamics enables the emergence of a uniform BB pattern, which in turn ensures the aligned deployment of motile cilia necessary for effective directional fluid flow.
arXiv:2605.15453v1 Announce Type: new
Abstract: A $P_4$ is a chordless path on four vertices. A diamond is a graph obtained from a clique of size four by removing one edge of the clique. A paw is a graph obtained from a clique of size four by removing two adjacent edges of the clique. We prove that for a graph $H$, the class of graphs with no induced subdivision of $H$ has bounded clique-width if and only if $H$ is an induced subgraph of $P_4$, the paw, or the diamond. This answers a~question of Dabrowski, Johnson, and Paulusma.