New software automates analysis of lab-grown brain cells for drug testing
Researchers have released LUMIN, a free tool that automatically analyzes calcium imaging data from cultured neurons—work that previously required hours of manual effort. The software processes thousands of cells in 30 minutes on a laptop, potentially accelerating how pharma companies and biotech firms screen drugs for neurological diseases and validate disease models.
Originaltitel: LUMIN: an automated graphical analysis toolbox for high-throughput calcium imaging of in vitro neuronal cultures
Human pluripotent stem cell-derived in vitro models, combined with advances in high-content live-cell calcium imaging, provide a powerful platform for disease modeling and drug screening. However, most calcium imaging analysis tools have been developed for in vivo applications with suboptimal features for processing data from cultured neurons. Here, we present LUMIN (Live-cell User Module for Imaging and analysis of Neuronal activity), a scalable and broadly applicable software that integrates a graphical user interface with automated data analysis encompassing single-cell segmentation, signal extraction, and activity quantification. Two analysis modules for transiently active or quiescent stimuli-evoked neurons make it broadly applicable for cultures with distinct functional properties. Its efficient data processing and linear time complexity enable processing thousands of cells within hours (~ 7000 cells from ~ 70 recordings per 30 min) on a standard laptop. We demonstrate LUMIN’s performance on human stem cell-derived ventral midbrain neurons through two applications: (1) modulation of spontaneous activity with well-characterized pharmacological compounds, and (2) evoking responses in quiescent neurons upon compound stimulation.