Scientists map how cancer-linked molecules silence genes, opening drug targets
Researchers used advanced NMR techniques to reveal how microRNA-34a—a molecule often malfunctioning in cancers—physically reorganizes when it latches onto its targets. The finding provides a blueprint for designing drugs that could restore normal gene regulation in cancer cells, potentially offering a new treatment strategy for multiple cancer types.
Originaltitel: Elucidating microRNA-34a organisation within human Argonaute-2 by dynamic nuclear polarisation-enhanced magic angle spinning NMR
<p>Understanding mRNA regulation by microRNA (miR) relies on the structural understanding of the RNA-induced silencing complex (RISC). Here, we elucidate the structural organisation of miR-34a, which is de-regulated in various cancers, in human Argonaute-2 (hAgo2), the effector protein in RISC. This analysis employs guanosine-specific isotopic labelling and dynamic nuclear polarisation (DNP)-enhanced Magic Angle Spinning (MAS) NMR. Homonuclear correlation experiments revealed that the non-A-form helical conformation of miR-34a increases when incorporated into hAgo2 and subsequently bound to SIRT1 mRNA compared to the free miR-34a or the free mRNA:miR duplex. The C8–C1′ correlation provided a nucleotide-specific distribution of C2′- and C3′-endo sugar puckering, revealing the capture of diverse dynamic conformations upon freezing. Predominantly C3′-endo puckering was observed for the seed region, while C2′-endo conformation was found in the central region, with a mixture of both conformations elsewhere. These observations provide insights into the molecular dynamics underlying miR-mediated mRNA regulation and demonstrate that experiments conducted under cryogenic conditions, such as at 90 K, can capture and reveal frozen dynamic states, using methods like DNP-enhanced MAS NMR or Cryo-Electron Microscopy.</p>