Drug-resistant bacteria spreading in African hospitals, threatening sepsis treatment
Researchers identified a dangerous multidrug-resistant bacterium called Klebsiella variicola in sepsis patients across four Ethiopian hospitals, with strains carrying genes that neutralize multiple antibiotics. The finding signals an urgent infection control crisis in low-income healthcare systems and underscores the commercial need for new antibiotics and diagnostic tools to combat resistant pathogens.
Originaltitel: Genomic characterizations of <em>Klebsiella variicola</em>: emerging pathogens identified from sepsis patients in Ethiopian referral hospitals
<p>Healthcare in low- and middle-income countries is becoming problematic due to the emergence of multidrug-resistant bacteria causing serious morbidity and mortality. <em>Klebsiella variicola</em> carrying multiple antimicrobial resistance (AMR) genes were found significantly among sepsis patients in a study done between October 2019 and September 2020 at four Ethiopian hospitals located in the central (Tikur Anbessa and Yekatit 12), southern (Hawassa), and northern (Dessie) parts. Among 1416 sepsis patients, 74 <em>K. variicola</em> isolates were identified using MALDI-TOF, most of them at Dessie (<em>n</em> = 44) and Hawassa (<em>n</em> = 28) hospitals. Whole genome sequencing showed that <em>K. variicola</em> strains identified at Dessie Hospital displayed phylogenetic clonality, carried an IncM1 plasmid and the majority were ST3924. Many <em>K. variicola</em> identified at Hawassa Hospital were clonally clustered and the majority belonged to novel STs and carried IncFIB(K) and IncFII(K) plasmids concurrently. Fifty <em>K. variicola</em> carried ESBL genes while 2 isolates harboured AmpC. Other frequently found genes were <em>aac(3)-lla, bla</em><sub>CTX-M-15</sub>, <em>bla</em><sub>TEM-1B</sub>, <em>bla</em><sub>LEN2,</sub> <em>bla</em><sub>OXA-1</sub>, <em>bla</em><sub>SCO-1</sub>, <em>catB3</em>, <em>dfrA14</em>, <em>QnrB1</em>, <em>aac(6’)-lb-cr</em> and <em>sul2</em>. Virulence genes detected at both sites were <em>mrk operons</em> for biofilm formation and siderophore ABC transporter operons for iron uptake. Capsular alleles varied, with <em>wzi 269</em> at Dessie and <em>wzi 582</em> at Hawassa. The isolation of multidrug-resistant <em>K. variicola</em> as an emerging sepsis pathogen calls for strong infection prevention strategies and antimicrobial stewardship supported by advanced bacterial identification techniques.</p>