New method unlocks gene analysis from tiny cell samples
Researchers have developed a faster, cheaper way to sequence genes from extremely small numbers of cells—even just lysed cell material. The technique could accelerate drug development, diagnostics, and basic research by making it practical to study individual cells or scarce tissue samples that were previously too difficult to analyze.
Originaltitel: T-RHEX-RNAseq - a tagmentation-based, rRNA blocked, random hexamer primed RNAseq method for generating stranded RNAseq libraries directly from very low numbers of lysed cells
ABSTRACT Background RNA sequencing has become the mainstay for studies of gene expression. Still, analysis of rare cells with random hexamer priming – to allow analysis of a broader range of transcripts – remains challenging. Results We here describe a tagmentation-based, rRNA blocked, random hexamer primed RNAseq approach (T-RHEX-RNAseq) for generating stranded RNAseq libraries from very low numbers of FACS sorted cells without RNA purification steps. Conclusion T-RHEX-RNAseq provides an easy-to-use, time efficient and automation compatible method for generating stranded RNAseq libraries from rare cells.