Dogs' genetic switches reveal how cells turn genes on and off instantly
Researchers mapped the precise molecular machinery controlling gene activity in dog cells, discovering that cells reprogram their entire genetic response within minutes of stress. The findings could accelerate drug development and disease modeling by clarifying how genes are regulated—insights applicable to human biology and personalized medicine.
Originaltitel: Transcriptional architecture and Pol II regulation at promoters, enhancers, and enhancer clusters in Canis lupus familiaris
<p>Domestic dog exhibits remarkable phenotypic diversity and provides versatile models for genomics, evolution, and complex traits. Genome sequencing and analyses of RNA expression have revealed regulatory regions in the dog genome. However, transcriptional activity, regulatory architecture, and control of RNA polymerase II (Pol II) across genes and enhancers remain uncharacterized. Here, we track transcription at nucleotide resolution, measure RNA expression and stability, and analyze mechanisms of Pol II regulation in golden retriever macrophages. We report precise architectures of promoters, enhancers, and enhancer clusters and quantify Pol II progression from the initiation, through pause region, into elongation and termination. Triggering transcriptional change by heat stress reveals instant reprogramming of genes via promoter-proximal pause regulation and enhancers via initiation. Enhancers within a cluster respond in unison. This study identifies functional genomic regions; characterizes transcriptional architectures of genes, enhancers, and enhancer clusters; quantifies RNA synthesis and stability; and reveals mechanisms of transcription in Canis lupus familiaris.</p>